Brefeldin A inhibits the antiviral action of interferon against encephalomyocarditis virus
Identifieur interne : 001246 ( Main/Exploration ); précédent : 001245; suivant : 001247Brefeldin A inhibits the antiviral action of interferon against encephalomyocarditis virus
Auteurs : G. S. Sidhu [États-Unis] ; A. K. Singh [Inde] ; P. N. Raghunath [États-Unis] ; S. Sivaram [Inde] ; R. K. Maheshwari [États-Unis]Source :
- Virus Research [ 0168-1702 ] ; 1996.
English descriptors
- Teeft :
- Antiviral, Antiviral action, Antiviral activity, Assay, Biol, Brefeldin, Cellular extracts, Dependent rnase, Dos, Emcv, Endoplasmic reticulum, Housekeeping gene, Hovanessian, Hybridization, Interferon, Internal control, Intracellular transport, Kinase, Lysis buffer, Northern hybridization, Nuclease, Phosphorylation, Poly, Present study, Primer, Protein kinase, Protein synthesis, Replication, Rnase, Secretory proteins, Sidhu, Silverman, Supernatant, Synthetase, Uninfected, Uninfected cells, Untreated control cells, Various concentrations, Various doses, Virol, Virus research.
Abstract
Abstract: Brefeldin A (BFA), a unique fungal metabolite of a 13-membered lactone ring, exhibits various biological actions, including antitumor, antifungal and antiviral activities. In the present study, mouse LB cells were treated with various concentrations of interferon (IFN) and/or BFA overnight and infected with encephalomyocarditis virus (EMCV) after removal of IFN and BFA. Doses of BFA which neither inhibit the metabolism of the cell nor the infectivity of EMCV, decreased the IFN-induced antiviral activity against EMCV as demonstrated by virus titer from supernatants. Since 2–5A synthetase and double-stranded RNA (dsRNA)-dependent protein kinase (PKR) have been suggested to be involved in the antiviral action of IFN against EMCV, their activities were investigated in LB cells after BFA treatment. Northern blot analysis and in situ hybridization showed a decrease (2–3-fold) in the mRNA of 2′–5′ oligoadenylate (2–5A) synthetase after BFA treatment. BFA also inhibited the activity of 2–5A synthetase, 2–5A dependent RNase and phosphorylation of PKR in cellular extracts, indicating that BFA may be exerting its inhibitory effect both at the transcriptional and post-transcriptional levels. This study reports a new biological action of BFA, demonstrating that BFA antagonized the antiviral action of IFN by inhibiting IFN-induced enzymatic pathways. These studies also suggest that both 2–5A and PKR are important in the antiviral activity of IFN against EMCV.
Url:
DOI: 10.1016/0168-1702(95)01262-1
Affiliations:
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S." last="Sidhu">G. S. Sidhu</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, Uniformed Services University of Health Sciences, Bethesda, MD 20814-4799</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Singh, A K" sort="Singh, A K" uniqKey="Singh A" first="A. K." last="Singh">A. K. Singh</name><affiliation wicri:level="1"><country xml:lang="fr">Inde</country><wicri:regionArea>Birla Institute of Technology and Science, Pilani Rajasthan</wicri:regionArea><wicri:noRegion>Pilani Rajasthan</wicri:noRegion></affiliation></author><author><name sortKey="Raghunath, P N" sort="Raghunath, P N" uniqKey="Raghunath P" first="P. N." last="Raghunath">P. N. 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Maheshwari</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology, Uniformed Services University of Health Sciences, Bethesda, MD 20814-4799</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Virus Research</title><title level="j" type="abbrev">VIRUS</title><idno type="ISSN">0168-1702</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996">1996</date><biblScope unit="volume">40</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="123">123</biblScope><biblScope unit="page" to="133">133</biblScope></imprint><idno type="ISSN">0168-1702</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0168-1702</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Antiviral</term><term>Antiviral action</term><term>Antiviral activity</term><term>Assay</term><term>Biol</term><term>Brefeldin</term><term>Cellular extracts</term><term>Dependent rnase</term><term>Dos</term><term>Emcv</term><term>Endoplasmic reticulum</term><term>Housekeeping gene</term><term>Hovanessian</term><term>Hybridization</term><term>Interferon</term><term>Internal control</term><term>Intracellular transport</term><term>Kinase</term><term>Lysis buffer</term><term>Northern hybridization</term><term>Nuclease</term><term>Phosphorylation</term><term>Poly</term><term>Present study</term><term>Primer</term><term>Protein kinase</term><term>Protein synthesis</term><term>Replication</term><term>Rnase</term><term>Secretory proteins</term><term>Sidhu</term><term>Silverman</term><term>Supernatant</term><term>Synthetase</term><term>Uninfected</term><term>Uninfected cells</term><term>Untreated control cells</term><term>Various concentrations</term><term>Various doses</term><term>Virol</term><term>Virus research</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Brefeldin A (BFA), a unique fungal metabolite of a 13-membered lactone ring, exhibits various biological actions, including antitumor, antifungal and antiviral activities. In the present study, mouse LB cells were treated with various concentrations of interferon (IFN) and/or BFA overnight and infected with encephalomyocarditis virus (EMCV) after removal of IFN and BFA. Doses of BFA which neither inhibit the metabolism of the cell nor the infectivity of EMCV, decreased the IFN-induced antiviral activity against EMCV as demonstrated by virus titer from supernatants. Since 2–5A synthetase and double-stranded RNA (dsRNA)-dependent protein kinase (PKR) have been suggested to be involved in the antiviral action of IFN against EMCV, their activities were investigated in LB cells after BFA treatment. Northern blot analysis and in situ hybridization showed a decrease (2–3-fold) in the mRNA of 2′–5′ oligoadenylate (2–5A) synthetase after BFA treatment. BFA also inhibited the activity of 2–5A synthetase, 2–5A dependent RNase and phosphorylation of PKR in cellular extracts, indicating that BFA may be exerting its inhibitory effect both at the transcriptional and post-transcriptional levels. This study reports a new biological action of BFA, demonstrating that BFA antagonized the antiviral action of IFN by inhibiting IFN-induced enzymatic pathways. These studies also suggest that both 2–5A and PKR are important in the antiviral activity of IFN against EMCV.</div></front></TEI><affiliations><list><country><li>Inde</li><li>États-Unis</li></country><region><li>Maryland</li></region></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Sidhu, G S" sort="Sidhu, G S" uniqKey="Sidhu G" first="G. S." last="Sidhu">G. S. Sidhu</name></region><name sortKey="Maheshwari, R K" sort="Maheshwari, R K" uniqKey="Maheshwari R" first="R. K." last="Maheshwari">R. K. Maheshwari</name><name sortKey="Raghunath, P N" sort="Raghunath, P N" uniqKey="Raghunath P" first="P. N." last="Raghunath">P. N. Raghunath</name></country><country name="Inde"><noRegion><name sortKey="Singh, A K" sort="Singh, A K" uniqKey="Singh A" first="A. K." last="Singh">A. K. Singh</name></noRegion><name sortKey="Sivaram, S" sort="Sivaram, S" uniqKey="Sivaram S" first="S." last="Sivaram">S. Sivaram</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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